Coding

Part:BBa_K4585002:Design

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-09-22)


VP64


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 138
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 138
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.


Source

VP64 is a transcription activation factor, originates from Vibrio parahaemolyticus. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and combine with UAS, to locate UAS.

References